Gpat4^{Gt(DTM030)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Gpat4^{Gt(DTM030)Byg}
• Name: glycerol-3-phosphate acyltransferase 4; gene trap DTM030, BayGenomics
• MGI ID: MGI:3624276
• Synonyms: Agpat6^-, Agpat6^{Gt(pGT1dTMpfs)1Byg}
• Gene: Gpat4 Location: Chr8:23661281-23698362 bp, - strand Genetic Position: Chr8, 11.42 cM
• Alliance: Gpat4^{Gt(DTM030)Byg} page

Mutation origin

• Mutant Cell Line: DTM030
• Germline Transmission: Earliest citation of germline transmission: J:108166
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1dTMpfs Vector Type: secretory trap
• Mutation details: A gene trap vector, pGT1dMpfs, containing a Betageo was inserted into intron 2 of the locus. Northern blot demonstrated lack of transcript in mutants. The insertion resulted in the production of an in-frame fusion transcript consisting of exons 1 and 2 from the locus and Betageo. (J:108166)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  DTM030	CC156158 ; (MGI Seq Detail)	5' RACE	Chr8:23680731-23681040 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  14 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	16 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpat4 Mutation: 34 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:108166 Beigneux AP, et al., Agpat6--a novel lipid biosynthetic gene required for triacylglycerol production in mammary epithelium. J Lipid Res. 2006 Apr;47(4):734-44
• All: 11 reference(s)