Rb1^tm1Fad
Targeted Allele Detail

Summary

• Symbol: Rb1^tm1Fad
• Name: RB transcriptional corepressor 1; targeted mutation 1, Frederick A Dick
• MGI ID: MGI:3624796
• Synonyms: Rb1^deltaL, Rb1^deltaLXCXE, Rb1^tm1Plam
• Gene: Rb1 Location: Chr14:73430298-73563446 bp, - strand Genetic Position: Chr14, 38.73 cM, cytoband D3
• Alliance: Rb1^tm1Fad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:108366
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: A targeting vector was used to insert I740A, N744A, M748A point mutations in the LXCXE binding site. Sequencing of exon 22 confirmed successful recombination. Analysis of mutant fibroblasts confirmed that protein levels were equivalent to wild-type cells. Crossing with mice expressing cre recombinase in the germline removed a floxed neo included in the vector. These substitutions are predicted to make the leucine and cysteine residues of the LXCXE motif a looser fit than the endogenous cleft. (J:108366, J:151417)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rb1 Mutation: 111 strains or lines available

References

• Original: J:108366 Isaac CE, et al., The retinoblastoma protein regulates pericentric heterochromatin. Mol Cell Biol. 2006 May;26(9):3659-71
• All: 15 reference(s)