Trpa1^tm1Kykw
Targeted Allele Detail

Summary

• Symbol: Trpa1^tm1Kykw
• Name: transient receptor potential cation channel, subfamily A, member 1; targeted mutation 1, Kelvin Y Kwan
• MGI ID: MGI:3624810
• Synonyms: Trpa1 KO
• Gene: Trpa1 Location: Chr1:14942872-14989086 bp, - strand Genetic Position: Chr1, 4.5 cM
• Alliance: Trpa1^tm1Kykw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:108343
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The S5 and S6 transmembrane domains and the pore loop containing the selectivity filter of the gene were replaced with an IRES-PLAP-pA cassette. The resulting sequence produces a bicistronic transcript containing the 5' end of the endogenous gene followed by an independently translated human placental alkaline phosphatase gene. An endoplasmic reticulum retention signal encoded by the amino acid sequence KDEL and a stop codon were inserted in frame with the exon before the IRES-PLAP to prevent unwanted side effects from a possibly truncated product of the endogenous coding sequence. RT-PCR confirmed absence of transcript in mutants. (J:108343)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trpa1 Mutation: 94 strains or lines available

References

• Original: J:108343 Kwan KY, et al., TRPA1 contributes to cold, mechanical, and chemical nociception but is not essential for hair-cell transduction. Neuron. 2006 Apr 20;50(2):277-89
• All: 142 reference(s)