Slc39a1^tm1.1Gka
Targeted Allele Detail

Summary

• Symbol: Slc39a1^tm1.1Gka
• Name: solute carrier family 39 (zinc transporter), member 1; targeted mutation 1.1, Glen K Andrews
• MGI ID: MGI:3625131
• Synonyms: ZIP1^-, ZIP1^- post-Cre
• Gene: Slc39a1 Location: Chr3:90155512-90160923 bp, + strand Genetic Position: Chr3, 39.21 cM
• Alliance: Slc39a1^tm1.1Gka page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:108627
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The enhanced green fluorescent protein (EGFP) coding sequence, followed by a termination codon, a polyadenylation signal and a loxP-flanked MC1-neo cassette, was inserted into the gene immediately following and in frame with the translation initiation codon in the second exon. The neo cassette was subsequently deleted by cre recombinase. EGFP is expressed under control of the native promoter. Absence of expression of the endogenous gene was demonstrated by Northern blot analysis, using a Slc39a1 cDNA probe, of total RNA and immunoblot analysis, using a polyclonal rabbit antiserum to a SLC38A1 peptide, of membrane proteins isolated from small intestine, kidney and liver of homozygous mutant mice. (J:108627)

Expression

In Mice Carrying this Mutation:	42 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc39a1 Mutation: 22 strains or lines available

Notes

Subjects and controls were day E14 embryos from homozygous mutant or wild-type matings whose dams were fed a zinc-deficient diet from day 8 of pregnancy.

Double mutant subjects were day E14 embryos from matings of mice homozygous for both mutations whose dams were fed a zinc-deficient diet from day 8 of pregnancy.

References

• Original: J:108627 Dufner-Beattie J, et al., Mouse ZIP1 and ZIP3 genes together are essential for adaptation to dietary zinc deficiency during pregnancy. Genesis. 2006 May;44(5):239-51
• All: 4 reference(s)