Tlr4^lps-2J
Spontaneous Allele Detail

Summary

• Symbol: Tlr4^lps-2J
• Name: toll-like receptor 4; defective lipopolysaccharide response 2 Jackson
• MGI ID: MGI:3626283
• Gene: Tlr4 Location: Chr4:66745788-66765338 bp, + strand Genetic Position: Chr4, 34.66 cM
• Alliance: Tlr4^lps-2J page

Mutation origin

• Strain of Origin: BXD29-Tlr4^lps-2J/J

Mutation description

• Allele Type: Spontaneous
• Mutation: Insertion
• Mutation details: Attempts to amplify the segment of exon 3 from the C57BL/6J DNA in the BXD29/TyJ strain representing nucleotides 279 - 708 of the cDNA sequence led to the discovery, near the 3' end of the gene, of a large insertion composed primarily of a repetitive sequence which exists in ~ 200 locations throughout the mouse genome. The insertion also includes a 239-bp sequence identical to a segment of Chr 10 between nucleotides 51,365,897 and 51,366,135 (build not identified). None of the endogenous gene is deleted, but the insertion is flanked at both ends by the same 8-nucleotide sequence (TCTTTCTT). Quantitative RT-PCR analysis detects no reduction in transcribed mRNA in peritoneal macrophages from mutant mice; however, these cells are not stained by antibodies against the encoded protein. (J:107391)
• Inheritance: Recessive

Expression

In Mice Carrying this Mutation:

4 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tlr4 Mutation: 91 strains or lines available

Notes

Recombinant inbred strain BXD29 carries this allele.

References

• Original: J:107391 Cook DN, et al., Spontaneous mutations in recombinant inbred mice: mutant toll-like receptor 4 (Tlr4) in BXD29 mice. Genetics. 2006 Mar;172(3):1751-5
• All: 8 reference(s)