Prkra^tm1Gcs
Targeted Allele Detail

Summary

• Symbol: Prkra^tm1Gcs
• Name: protein kinase, interferon inducible double stranded RNA dependent activator; targeted mutation 1, Ganes C Sen
• MGI ID: MGI:3626341
• Synonyms: Pact^-
• Gene: Prkra Location: Chr2:76460242-76478359 bp, - strand Genetic Position: Chr2, 45.07 cM
• Alliance: Prkra^tm1Gcs page

External and internal ear abnormalities in Prkra^tm1Gcs/Prkra^tm1Gcs mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:108297
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace about 500 bp of sequence encoding the PKR activation domain in exon 8 with a neomycin resistance cassette. No truncated protein was expressed. The mutant hybrid mRNA was shown to contain 1-22 bases of the endogenous sequence, intron 7, 923-934 of the disrupted exon 8 and the entire neo cassette. (J:108297)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkra Mutation: 24 strains or lines available

References

• Original: J:108297 Rowe TM, et al., A role of the double-stranded RNA-binding protein PACT in mouse ear development and hearing. Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):5823-8
• All: 6 reference(s)