Mpi^{Gt(OST90588)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Mpi^{Gt(OST90588)Lex}
• Name: mannose phosphate isomerase; gene trap OST90588, Lexicon Genetics
• MGI ID: MGI:3628756
• Synonyms: Mpi^-, Mpi^{Gt(VICTR48)1Lex}
• Gene: Mpi Location: Chr9:57451539-57460046 bp, - strand Genetic Position: Chr9, 31.05 cM, cytoband C
• Alliance: Mpi^{Gt(OST90588)Lex} page

Mutation origin

• Mutant Cell Line: OST90588
• Germline Transmission: Earliest citation of germline transmission: J:109220
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The insertion of the VICTR48 vector into intron 1 resulted in the splicing of 5' exonic sequences to a neomycin resistance cassette. The vector contained long terminal repeats, neo-pA, BTK and additional LTR sequence. The final product was a fusion of exon 1 and the neo. Protein expression was not detected in mutant embryos. (J:109220)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST90588	CG521870 ; (MGI Seq Detail)	3' RACE	Chr9:57458014-57459492 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  7 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mpi Mutation: 32 strains or lines available

References

• Original: J:109220 DeRossi C, et al., Ablation of mouse phosphomannose isomerase (Mpi) causes mannose 6-phosphate accumulation, toxicity, and embryonic lethality. J Biol Chem. 2006 Mar 3;281(9):5916-27
• All: 2 reference(s)