Adam1b^tm1Tba
Targeted Allele Detail

Summary

• Symbol: Adam1b^tm1Tba
• Name: a disintegrin and metallopeptidase domain 1b; targeted mutation 1, Tadashi Baba
• MGI ID: MGI:3628759
• Synonyms: Adam1b^-
• Gene: Adam1b Location: Chr5:121638161-121641498 bp, - strand Genetic Position: Chr5, 61.83 cM
• Alliance: Adam1b^tm1Tba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:109221
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A vector was designed to replace the 455-residue protein-coding region containing the pro-metalloprotease, disintegrin, and cysteine-rich domains at positions 79-533 with a neomycin resistance gene. Absense of transcript was confirmed in Northern blot of mutant testis. Also, protein extracts from testicular germ cells lacked a 120-kDa protein corresponding to the precursor form of Adam1b protein. (J:109221)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Adam1b Mutation: 25 strains or lines available

References

• Original: J:109221 Kim E, et al., Mouse sperm lacking ADAM1b/ADAM2 fertilin can fuse with the egg plasma membrane and effect fertilization. J Biol Chem. 2006 Mar 3;281(9):5634-9
• All: 1 reference(s)