Pik3ca^tm1Bvan
Targeted Allele Detail

Summary

• Symbol: Pik3ca^tm1Bvan
• Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; targeted mutation 1, Bart Vanhaesebroeck
• MGI ID: MGI:3628805
• Synonyms: p110alpha^D933A, p110alpha KI
• Gene: Pik3ca Location: Chr3:32451203-32520256 bp, + strand Genetic Position: Chr3, 15.7 cM, cytoband B
• Alliance: Pik3ca^tm1Bvan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:109024
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: This allele is derived from Pik3ca and was produced as follows: A point mutation was engineered into the locus, resulting in the DFG to AFG conversion in the conserved DFG motif at the C-terminal kinase domain. A C-terminal Myc-epitope tag was introduced immediately preceding the translational stop codon as well as a floxed IRES-lacZ-geo cassette in the 3' UTR. The floxed sequence was subsequently removed by crossing with mice carrying the Tg(CMV-cre)1Cgn transgene. The p110alpha, p110beta, and p110gamma subunit expression was not altered in homozygous embryos and in liver, muscle and fat from hetrozygous mice. The heterozygous mutant demonstrated 50% loss of function in overall class IA PI(3)K activity in liver, muscle and fat. (J:109024)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pik3ca Mutation: 66 strains or lines available

References

• Original: J:109024 Foukas LC, et al., Critical role for the p110alpha phosphoinositide-3-OH kinase in growth and metabolic regulation. Nature. 2006 May 18;441(7091):366-70
• All: 14 reference(s)