Kdr^tm2.1Jrt
Targeted Allele Detail

Summary

• Symbol: Kdr^tm2.1Jrt
• Name: kinase insert domain protein receptor; targeted mutation 2.1, Janet Rossant
• MGI ID: MGI:3629041
• Synonyms: Flk1-EGFP(neo out), Flk1-GFP, VEGFR2-GFP
• Gene: Kdr Location: Chr5:76093487-76139118 bp, - strand Genetic Position: Chr5, 40.23 cM
• Alliance: Kdr^tm2.1Jrt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:109435
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An in-frame EGFP cDNA and a loxP site flanked neomycin resistance gene cassette replaced the translated portion of the first coding exon and the proximal part of the next intron, and placed EGFP under the transcriptional control of the endogenous promoter. The floxed neo cassette was subsequently removed by crossing to a cre-deleter strain. (J:109435)

Expression

In Mice Carrying this Mutation:	11 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Kdr Mutation: 74 strains or lines available

References

• Original: J:109435 Ema M, et al., Deletion of the selection cassette, but not cis-acting elements, in targeted Flk1-lacZ allele reveals Flk1 expression in multipotent mesodermal progenitors. Blood. 2006 Jan 1;107(1):111-7
• All: 35 reference(s)