Dph3^tm1Lepp
Targeted Allele Detail

Summary

• Symbol: Dph3^tm1Lepp
• Name: diphthamine biosynthesis 3; targeted mutation 1, Stephen H Leppla
• MGI ID: MGI:3629060
• Synonyms: dph3^-
• Gene: Dph3 Location: Chr14:31802523-31807686 bp, - strand Genetic Position: Chr14, 19.4 cM
• Alliance: Dph3^tm1Lepp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:109066
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace the first two exons and a small part of exon 3 with a neomycin resistance cassette. RT-PCR of mutant ES cells confirmed absence of transcript. (J:109066)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dph3 Mutation: 12 strains or lines available

References

• Original: J:109066 Liu S, et al., Dph3, a small protein required for diphthamide biosynthesis, is essential in mouse development. Mol Cell Biol. 2006 May;26(10):3835-41
• All: 1 reference(s)