Fatpad3^CAST/EiJ
QTL Variant Detail

Summary

• QTL variant: Fatpad3^CAST/EiJ
• Name: fat pad 3; CAST/EiJ
• MGI ID: MGI:3629159
• QTL: Fatpad3 Location: unknown Genetic Position: Chr13, Syntenic

Variant origin

• Strain of Specimen: CAST/EiJ

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased fat pad weights compared to M16i. (J:108423)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:108423

Linkage analysis was performed on 421 animals from a M16i x (M16i x CAST/EiJ)F1 backcross to identify QTL associated with body weight and body composition. 92 microsatellite markers were used for the genome scan. Parental strain M16i exhibits increased growth rate, body weight, and mild obesity compared to parental strain CAST/EiJ. All identified QTL appear to participate in epistatic interactions contributing a low but detectable percentage of the phenotypic variance.

Linkage to body weight at 3 weeksof age mapped to 72 cM on mouse Chromosome 1 (LOD=5.74 near D1Mit140) and is named Gwth1 (growth traits 1). The confidence interval spans 57 cM - 86 cM. Gwth1 participates in epistatic interaction with the locus on chromosome 18 at D18Nds1 (Gwth7) affecting fatness and body weight in older mice. Gwth1 is also linked to kidney weight with LOD=6.89.

Linkage to body weight mapped to several peaks on mouse Chromosome 2 and is collectively called Gwth2 (growth traits 2). The confidence interval of Gwth2 spansapproximately 36 cM - 85 and is linked to body weight at 6, 9, and 12 (highest LOD=13.65). Linkage to gonadal fat pad weight mapped to 77 cM (LOD=17-20) and is named Fatpad1. This locus explains 18% of the fat trait variance and coincides with a QTL peakfor body weight at 9 and 12 weeks. Linkage to liver weight mapped to 64 cM with LOD=17.61. This locus is named Orgwt1 (organ weight 1). The confidence interval of Orgwt1 spans 59 cM - 69 cM. Linkage to spleen weight and kidney weight occurred at 73 cM (Orgwt2; LOD=3.97) and 86 cM (Orgwt3; LOD=8.49), respectively.

Linkage to kidney weight was detected at 65 cM on mouse Chromosome 3 (LOD=3.54). The confidence interval spans 47 cM - 75 cM. This locus is named Orgwt4 (organ weight 4).

Linkage to body weightat 12 days of age mapped to 17 cM on mouse Chromosome 4 (LOD=3.41). This locus is named Gwth3 (growth traits 3). The confidence interval spans 11 cM - 41 cM. Linkage to spleen weight mapped to 49 cM with LOD=6.51. This locus is named Orgwt5 (organ weight5).

Linkage to combined fat pad weight mapped to 31 cM on mouse Chromosome 5 with LOD=3.63. This locus is named Fatpad2. The confidence interval of Fatpad2 spans 12 cM - 28 cM.

Linkage to spleen weight mapped to 28 cM on mouse Chromosome 9 with LOD=7.87. This locus is named Orgwt6 (organ weight 6). The confidence interval spans 17 cM - 40 cM.

Linkage to spleen weight mapped to 29 cM on mouse Chromosome 10 with LOD=3.54. This locus is named Orgwt7 (organ weight 7). The confidence interval spans 20cM - 40 cM.

On mouse Chromosome 11, linkage to liver weight and body weight at 12 weeks of age mapped to 23 cM with LOD=4.14. This locus is named Gwth4 (growth traits 4). The confidence interval spans 7 cM - 52 cM.

Linkage to fat traits mapped to 34 cM-41cMon mouse Chromosome 13 (highest LOD=4.6) and is named Fatpad3. The confidence interval spans approximately 21 cM - 47 cM. CAST/EiJ-derived alleles at Fatpad3 confer increased fat traits (gonadal, subcutaneous, and combined). This locus explains 4% of thefat trait variance.

Linkage to subcutaneous fat pad weight mapped to 30 cM on mouse Chromosome 14 with LOD=3.7. This locus is named Fatpad4. The confidence interval of Fatpad4 spans 16 cM - 43 cM.

On mouse Chromosome 15, linkage to body weightat 9 and 12weeks of age mapped to an interval from 42 cM - 58 cM (highest LOD=4.16). This locus is named Gwth 5 (growth traits 5). The confidence interval spans approximately 32 cM - 62 cM. Linkage to all fat pad traits (gonadal, subcutaneous, and combined)mappedto35 cM (highest LOD=4.54). This locus is named Fatpad5. Linkage to gondal and combined fat pad weights mapped to 24 cM (highest LOD= 3.63). This locus is named Fatpad6.

Linkage to gonadal fat pad weight mapped to 22 cM on mouse Chromosome 17with LOD=3.32.This locus is named Fatpad7.

On mouse Chromosome 18, linkage to body weight at 12 days of age mapped to 22 cM with LOD=3.34. This locus is named Gwth6 (growth traits 6). The confidence interval spans 6 cM - 72 cM. A second locus named Gwth7 (growthtraits7) mapped to 45 cM - 48 cM. Gwth7 is linked to body weight at 12 days and 3 weeks of age with maximum LOD=4.71 near D18Nds1. The confidence interval of Gwth7 spans 13 cM - 65 cM. This locus participates in epistatic interaction with Gwth1 on chromosome 1and affects fatness and body weight in older mice. A locus named Orgwt8 (organ weight 8) at 73 cM shows linkage to kidney weight with LOD=6.95. The confidence interval spans 61 cM - 73 cM.

J:152067

To determine QTLs effecting body weight C57BL/6J mice were mated to PWK/PhJ and the resulting F2 hybrids were used in mapping. A total of 479 F2 mice (235 female and 244 male) were bred from two types of reciprocal crosses: 236 and 243 F2 mice were obtained from (B6 female X PWK male) F1 Female X (B6 female X PWK male) F1 male crosses and (PWK female X B6 male) F1 female with (B6 female X PWK male) F1 male crosses respectively.

Four phenotypes were analyzed, these were body weight, lean weight,fat weight, and percent fat (fat weight/body weight X 100). C57BL/6J mice are heavier then PWK/PhJ mice.

The following new and existing QTLs were mapped

(following the QTL is the linked peak marker with cM locations):

Fatq1(D1Mit440 - 54 cM),

Pbwg1.1(D2Mit285 - 86 cM),

Adip22 (was identified as Adip10)(D2Mit293 -11cM),

Gnf5 (D2Mit285 - 86 cM),

Wt10q4 (was identified as Wt10q2) (D3Mit57 - 55 cM),

Btwq13 (D3Mit5 - 25cM),

Afw13 (was identified as Afw1) (D3Mit5 - 25 cM),

Afpq8 (was identified as Afpq1)(D3Mit256 - 66cM),

Bw6b (D3Mit17 - 32 cM),

Epb4.1 (D4Mit17 - 32 cM),

Adip23 (was identified as Adip11)Adip11 (D4Mit17 -32 cM),

Bwq8 (D5Mit123- 3cM),

Bwtq14 (was identified as Bw6d and Bwob) (rs3719351 - 49 cM),

Afw3 (D5Mit314 - 59 cM),

Bwtq7 (was identified as Bwtq10)(D6Mit198 - 67 cM),

Pfat5 (D6Mit284 - 38 cM),

Pbwg3(D7Mit223 - 72 cM),

Mtbcq9 (was identified as Mtbcq8)(D9Mit129 - 26 cM),

Adip21 (was identified as Adip5)(D9Mit123 - 42 cM),

Adip24 (was identified as Adip15)(D10Mit96 - 56 cM),

Bw4(D11Mit285 -48 cM),

Adip19 (D11Mit285 - 52 cM),

Fatpad3 ( D13Mit144 - 48 cM),

Bw40 (was identified as Bw10) (D13Mit144 - 48 cM),

Fatq1 (D16Mit107 - 3 cM)'

and Adip25 (was identified as Adip17) (D16Mit107 - 3 cM).

The new QTLs mapped in this study were:

Bwtq13,

Bwq13 (alias Bwq8),

Bwq9 (alias Pbwq1.1),

Bwq11 (alias Pbwq3),

Pfat5 (was identified as Pfat2),

Gnf5 (was identified as Gnf3)

and Adip19.

10.03.2014 Curator Note:

We had originally associated the abdominal fatweight QTL identified here mapping to (D3Mit5- 25 cM) on mouse Chr 3, as Afw1. Afw1 was originally mapped using an outbred cross of DU6 xDUK, in J:49578 in 1998. We consider the current mapping study a separate mapping experiment and have named the QTL identified here as Afw13.

01.07.2015 Curator Note:

We had originally associated the QTL identified here, mapping to D13Mit144 - 48 cM as Bw10. However, Bw10 was originally mapped using a different cross, an outbred cross of DU6 x DUK, in J:49578, in 1998. We consider the current mapping study aseparate mapping experiment and have named the QTL identified here as Bw40.

01.23.2015 Curator Note:

Because Adip5 was origniallymapped in J:62610 in 2001 using an (LG/J x SM/J) intercross, which differs from the cross used here, we consider the current study a separate mapping experiment and have named this QTL Adip21.

Adip10, Adip11, Adip15 and Adip17 mentioned here were all originally mapped in J:103841 in 2006 using an (SM/J x NZB/BlNJ)F2 intercross, whichalsodiffers from the cross used here. For thesame reasons noted above we have named these markers Adip22, Adip23, Adip24 and Adip25 respectively.

03.11.2015 Curator Note:

Because Bwtq10 was originally mapped in J:103841 in 2006 using (SM/J x NZB/BlNJ)F2 intercross, which differs from thecross usedhere, we consider the current study a separate map experiment and have named this QTL Bwqt7, (D6Mit198- 67 cM).

04.2.2015 Curator Note:

Mtbcq8 was originally mapped in J:93070 in 2004 using an (NMRI8/Fbn xDBA/2OlaHsd)F2 intercross, which differs fromthe cross used here, we consider the current study a new map experiment and have named the QTL Mtbcq9 (D9Mit129 - 26cM).

04.14.2015 Curator Note:

Because the abdominal fat percent QTL 1, (Afpq1) was orginally mapped in J:49578 in 1998 using an an outbredcross of DU6 xDUK, which differs from the cross used here we consider the current mapping study a separate mapping experiment and have named the QTL Afpq8.

04.15.2015 Curator Note:

Because Wt10q2 was originally mapped in J:55483 in 1999 using an (MHxC57BL/6J)F2 intercross, which differs from the cross used here, we consider the current mapping study a separate mapping experiment and have named the QTL Wt10q4.

06.16.2015 Curator Note:

Pbwq1.1 was orginally mapped in J:121981 using B6.Cg congenic mice, which differ from the C57BL/6J X PWK/PhJ mice used here. We consider the current mapping study a separate mapping experiment and have named this QTL, mapping to Chr 2 with D2Mit285, Bwq9.

Pbwq3 was originally mapped in J:88536 using an (C57BL/6J x M. m. castaneus)F1 x M. m. castaneus backcross, which also differs from the cross used here. We have named this QTL, mapping to Chr 7 with D7Mit223, Bwq11.

References

• Original: J:108423 Yi N, et al., Bayesian analyses of multiple epistatic QTL models for body weight and body composition in mice. Genet Res. 2006 Feb;87(1):45-60
• All: 1 reference(s)