Sohlh1^tm1Rajk
Targeted Allele Detail

Summary

• Symbol: Sohlh1^tm1Rajk
• Name: spermatogenesis and oogenesis specific basic helix-loop-helix 1; targeted mutation 1, Aleksandar Rajkovic
• MGI ID: MGI:3629211
• Synonyms: Sohlh1^-
• Gene: Sohlh1 Location: Chr2:25733007-25737260 bp, - strand Genetic Position: Chr2, 18.21 cM
• Alliance: Sohlh1^tm1Rajk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:109310
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exons 2-8 with a pgk-NPRT cassette. The deleted sequence included 500 nucleotides upstream of the putative transcriptional initiation site and the first three exons that encode the bHLH domain. Semi-quantitative RT-PCR confirmed absence of transcript in mutant testes. (J:109310)

Expression

In Mice Carrying this Mutation:	22 assay results 3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sohlh1 Mutation: 15 strains or lines available

References

• Original: J:109310 Ballow D, et al., Sohlh1 is essential for spermatogonial differentiation. Dev Biol. 2006 Jun 1;294(1):161-7
• All: 6 reference(s)