Trp53^tm2Xu
Targeted Allele Detail

Summary

• Symbol: Trp53^tm2Xu
• Name: transformation related protein 53; targeted mutation 2, Yang Xu
• MGI ID: MGI:3629221
• Synonyms: p53^S18/23A
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm2Xu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:109354
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Site-directed mutagenesis within exon 2 generated missense mutations at codons 18 and 23, resulting in the substitutions of both serines with alanines (p.S18A, p.S23A). A single loxP site remained in intron 4 after a loxP site flanked neomycin resistance gene cassette was excised via Cre-mediated recombination. Sequencing verified that only the S18/23A mutations were present in mutant mice. (J:109354)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm2Xu

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 239 strains or lines available

References

• Original: J:109354 Chao C, et al., Ser18 and 23 phosphorylation is required for p53-dependent apoptosis and tumor suppression. EMBO J. 2006 Jun 7;25(11):2615-22
• All: 3 reference(s)