Msn^tm1Sts
Targeted Allele Detail

Summary

• Symbol: Msn^tm1Sts
• Name: moesin; targeted mutation 1, Shoichiro Tsukita
• MGI ID: MGI:3630284
• Gene: Msn Location: ChrX:95139651-95212159 bp, + strand Genetic Position: ChrX, 42.65 cM
• Alliance: Msn^tm1Sts page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:52936
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 3'end of exon 3 and part of intron 3 was replaced with a loxP-flanked neomycin selection cassette. A splice acceptor (SA) and polyadenylation signal (PA) were also placed at the 3' end of the cassette to minimize the possibility of producing aberrant transcripts that skip exon 3. Immunoblotting assays on mutant male ES cells (-/Y) demonstrated that no full length protein was expressed from this allele. (J:52936)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Msn Mutation: 24 strains or lines available

References

• Original: J:52936 Doi Y, et al., Normal development of mice and unimpaired cell adhesion/cell motility/actin-based cytoskeleton without compensatory up-regulation of ezrin or radixin in moesin gene knockout. J Biol Chem. 1999 Jan 22;274(4):2315-21
• All: 10 reference(s)