Cd8a^tm1.1Kio
Targeted Allele Detail

Summary

• Symbol: Cd8a^tm1.1Kio
• Name: CD8 subunit alpha; targeted mutation 1.1, Dimitris Kioussis
• MGI ID: MGI:3630292
• Synonyms: deltaCII mutant
• Gene: Cd8a Location: Chr6:71350411-71356155 bp, + strand Genetic Position: Chr6, 32.14 cM, cytoband C
• Alliance: Cd8a^tm1.1Kio page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76799
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Two DNAaseI hypersensitive sites upstream of the Cd8a gene, HSS1 and HSS2, were deleted by homologous recombination. These sites are thought to contain regulatory sequences that control Cd8a expression. Targeting took place in ES cells homozygous for the Tg(Prm-cre)70Og transgene, which expresses Cre recombinase in male germ cells. An inserted loxP-flanked neomycin selection cassette was deleted by Cre-mediated recombination upon transmission from male chimeric mice. (J:76799)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd8a Mutation: 39 strains or lines available

References

• Original: J:76799 Garefalaki A, et al., Variegated expression of CD8 alpha resulting from in situ deletion of regulatory sequences. Immunity. 2002 May;16(5):635-47
• All: 1 reference(s)