Tg(Myh6-Prkaa2*D157A)1Rti
Transgene Detail

Summary

• Symbol: Tg(Myh6-Prkaa2*D157A)1Rti
• Name: transgene insertion 1, Rong Tian
• MGI ID: MGI:3639282
• Synonyms: AMPK-DN, TG^alpha2DN
• Transgene: Tg(Myh6-Prkaa2*D157A)1Rti Location: unknown
• Alliance: Tg(Myh6-Prkaa2*D157A)1Rti page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-Prkaa2*D157A)1Rti expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Prkaa2 (78975)

  Prkaa2

  (MGI:1336173)

  Transgene codes for a protein with an aspartic acid to alanine mutation.

• Mutation details: This transgene contains the full length cDNA of rat Prkaa2 with the aspartic acid at residue 157 changed to alanine to render the catalytic subunit inactive, tagged with an HA epitope, under the control of the cardiac specific mouse alpha-myosin heavy chain promoter. Prkaa2 activity is reduced in hearts at base line by 78%. (J:86014, J:97374)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:86014 Xing Y, et al., Glucose metabolism and energy homeostasis in mouse hearts overexpressing dominant negative alpha2 subunit of AMP-activated protein kinase. J Biol Chem. 2003 Aug 1;278(31):28372-7
• All: 14 reference(s)