Tg(Ckm-Prkaa2*K45R)1Mbb
Transgene Detail

Summary

• Symbol: Tg(Ckm-Prkaa2*K45R)1Mbb
• Name: transgene insertion 1, Morris J Birnbaum
• MGI ID: MGI:3639284
• Synonyms: alpha2 AMPK KD, Tg-KD1
• Transgene: Tg(Ckm-Prkaa2*K45R)1Mbb Location: unknown
• Alliance: Tg(Ckm-Prkaa2*K45R)1Mbb page

Transgene origin

• Strain of Origin: (C57BL/6J x SJL/J)F1/J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ckm-Prkaa2*K45R)1Mbb expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Prkaa2 (78975)

  Prkaa2

  (MGI:1336173)

  Transgene codes for a protein with a lysine to arginine mutation.

• Mutation details: This transgene contains the full length cDNA of rat Prkaa2 with the lysine at residue 45 (residue critical for ATP binding and hydrolysis) changed to arginine and tagged at the 5'end with a myc epitope under the control of the muscle creatine kinase promoter. Expression of endogenous Prkaa2 is completely undetectable and transgene expression occurs in skeletal and cardiac muscle. (J:110092)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:110092 Mu J, et al., A role for AMP-activated protein kinase in contraction- and hypoxia-regulated glucose transport in skeletal muscle. Mol Cell. 2001 May;7(5):1085-94
• All: 47 reference(s)