Tg(Myh6-EP300)21Csk
Transgene Detail

Summary

• Symbol: Tg(Myh6-EP300)21Csk
• Name: transgene insertion 21, Chugai Pharmaceutical Co, Ltd
• MGI ID: MGI:3640671
• Synonyms: line 21-TG, p300 TG, p300-TG
• Transgene: Tg(Myh6-EP300)21Csk Location: unknown
• Alliance: Tg(Myh6-EP300)21Csk page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-EP300)21Csk expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  EP300 (2033)

  Ep300

  (MGI:1276116)

• Mutation details: A full-length cDNA encoding human E1A binding protein p300 was ligated to the 5' end of a 5.5-kb fragment of mouse genomic DNA that includes the entire, promoter-containing intergenic region between the beta- (downstream) and alpha-cardiac myosin heavy chain genes. RT-PCR with transgene-specific primers detected mRNA expression in heart, but not in lung, liver or kidney. Immunoblot analysis detected 8-9-fold as much of the (endogenous plus transgene-encoded) protein in cardiac nuclear extracts from transgenic than from wild-type mice. (J:110549)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
Loading...
tg1	Tg(Myh6-EP300)21Csk/0	C57BL/6-Tg(Myh6-EP300)21Csk

Phenotypes:

Affected Systems	tg1
show or hide all annotated terms
cardiovascular system	√
abnormal myocardial fiber morphology	√
cardiac hypertrophy	√
dilated heart left ventricle	√
decreased heart left ventricle muscle contractility	√
congestive heart failure	√
growth/size/body	√
cardiac hypertrophy	√
homeostasis/metabolism	√
decreased susceptibility to xenobiotic induced morbidity/mortality	√
decreased physiological sensitivity to xenobiotic	√
mortality/aging	√
decreased susceptibility to xenobiotic induced morbidity/mortality	√
premature death	√
muscle	√
abnormal myocardial fiber morphology	√
decreased heart left ventricle muscle contractility	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

The original publication (J:110549) does not name or distinguish among three lines derived from independent transgenic founders. Kawamura et al. (J:110550) identify lines 21 and 39.

References

• Original: J:110549 Yanazume T, et al., Cardiac p300 is involved in myocyte growth with decompensated heart failure. Mol Cell Biol. 2003 May;23(10):3593-606
• All: 4 reference(s)