Ocln^{Gt(ROSA)39Tshi}
Gene trapped Allele Detail

Summary

• Symbol: Ocln^{Gt(ROSA)39Tshi}
• Name: occludin; gene trap 39, Takashi Shinohara
• MGI ID: MGI:3640976
• Gene: Ocln Location: Chr13:100633015-100689226 bp, - strand Genetic Position: Chr13, 53.23 cM
• Alliance: Ocln^{Gt(ROSA)39Tshi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:110222
• Parent Cell Line: Not Specified (Spermatogonial Stem Cell)
• Strain of Origin: DBA/2

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: Random insertion of retroviral gene trap vector ROSA, Reverse Orientation with Splice Acceptor, which contains the beta-galactosidase gene and accompanying splice acceptor and neo sequences. The construct was inserted in reverse orientation in the retroviral vector pGen-. RT-PCR and Western blot analysis confirmed the absence of mRNA and protein expression in homozygous lungs and kidneys. (J:110222)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ocln Mutation: 33 strains or lines available

References

• Original: J:110222 Kanatsu-Shinohara M, et al., Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells. Proc Natl Acad Sci U S A. 2006 May 23;103(21):8018-23
• All: 1 reference(s)