Polr2a^tm2Cdn
Targeted Allele Detail

Summary

• Symbol: Polr2a^tm2Cdn
• Name: polymerase (RNA) II (DNA directed) polypeptide A; targeted mutation 2, J L Corden
• MGI ID: MGI:3641215
• Synonyms: Rpo2-1^spe
• Gene: Polr2a Location: Chr11:69624823-69649459 bp, - strand Genetic Position: Chr11, 42.86 cM, cytoband B1-C
• Alliance: Polr2a^tm2Cdn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:53757
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance gene was inserted outside the transcribed region of the locus and a 13 repeat deletion inserted into the C-terminus. Western blot analysis indicated a smaller protein was present in mutants than in wild-types. (J:53757)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Polr2a Mutation: 92 strains or lines available

References

• Original: J:53757 Litingtung Y, et al., Growth retardation and neonatal lethality in mice with a homozygous deletion in the C-terminal domain of RNA polymerase II. Mol Gen Genet. 1999 Feb;261(1):100-5
• All: 1 reference(s)