Pparg^tm2Mae
Targeted Allele Detail

Summary

• Symbol: Pparg^tm2Mae
• Name: peroxisome proliferator activated receptor gamma; targeted mutation 2, Nobuyo Maeda
• MGI ID: MGI:3641248
• Synonyms: Pparg^c
• Gene: Pparg Location: Chr6:115337912-115467360 bp, + strand Genetic Position: Chr6, 53.41 cM, cytoband E3-F1
• Alliance: Pparg^tm2Mae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:110607
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: A 69 bp fragment containing an AU rich element was inserted into the 3' untranslated region downstream of the stop codon via homologous recombination. In addition a self-excising neo resistance cassette consisting of a floxed mouse testes-specific Ace promoter fused to the cre recombinase and neomycin resistance genes was also inserted and then removed during germline transmission. RT-PCR showed that message levels are reduced about 40 - 50% in adipose tissue, liver, kidney, and heart in homozygotes compared to wild-type mice. Mice generated from one targeted ES cell clone (YTC-1) also have a tightly linked mutation Atp2b2^m1Mae. (J:110607)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pparg Mutation: 41 strains or lines available

References

• Original: J:110607 Tsai YS, et al., A de novo deafwaddler mutation of Pmca2 arising in ES cells and hitchhiking with a targeted modification of the Pparg gene. Mamm Genome. 2006 Jul;17(7):716-22
• All: 4 reference(s)