Cast^tm1Tcs
Targeted Allele Detail

Summary

• Symbol: Cast^tm1Tcs
• Name: calpastatin; targeted mutation 1, Takaomi C Saido
• MGI ID: MGI:3641275
• Gene: Cast Location: Chr13:74840487-74956929 bp, - strand Genetic Position: Chr13, 40.52 cM, cytoband C1
• Alliance: Cast^tm1Tcs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:109976
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Most of exon 6 was replaced with a pgk-neo in reverse orientation. Northern and Western blot analysis confirmed recombination of the locus, as did RT-PCR. Calpastatin activity assays indicated that calpastatin activity was reduced to an undetectable level in the mutant mouse brain. (J:109976)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cast Mutation: 63 strains or lines available

References

• Original: J:109976 Takano J, et al., Calpain mediates excitotoxic DNA fragmentation via mitochondrial pathways in adult brains: evidence from calpastatin mutant mice. J Biol Chem. 2005 Apr 22;280(16):16175-84
• All: 13 reference(s)