Tg(Nodal-lacZ)8Hmd
Transgene Detail

Summary

• Symbol: Tg(Nodal-lacZ)8Hmd
• Name: transgene insertion 8, Hiroshi Hamada
• MGI ID: MGI:3641292
• Synonyms: 3'-1 transgene, A8 line, Tg(Nodal ASE-LacZ)8Hh
• Transgene: Tg(Nodal-lacZ)8Hmd Location: unknown
• Alliance: Tg(Nodal-lacZ)8Hmd page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F2

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgenic mouse line designated A8 was created using construct 3'1, which contains the 5' proximal promoter region of the Nodal locus extending from 5kb to 50 bp upstream of the translation initiation codon followed by the bacterial beta-galactosidase (lacZ) gene and a 3.5-kb segment of Nodal including intron 1, wherein resides a left side-specific asymmetric enhancer (ASE). Expression is observed throughout the epiblast of the embryo at E5.5, restricted to the proximal epiblast at E6.5, and in the anterior visceral endoderm from embryonic day E6.0 through E6.5. At the early somite stage, expression occurs on the left side of the lateral plate mesoderm (LPM) in the splanchopleure and somatopleure. (J:56146)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:56146 Adachi H, et al., Determination of left/right asymmetric expression of nodal by a left side-specific enhancer with sequence similarity to a lefty-2 enhancer. Genes Dev. 1999 Jun 15;13(12):1589-600
• All: 2 reference(s)