Ly86^tm1Kmiy
Targeted Allele Detail

Summary

• Symbol: Ly86^tm1Kmiy
• Name: lymphocyte antigen 86; targeted mutation 1, Kensuke Miyake
• MGI ID: MGI:3641422
• Synonyms: MD-1^-
• Gene: Ly86 Location: Chr13:37529276-37603012 bp, + strand Genetic Position: Chr13, 14.7 cM
• Alliance: Ly86^tm1Kmiy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:109720
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting construct was designed to replace exon 1 with a neomycin resistance gene. Exon 1 encodes the signal sequence and 27 amino acids of the expressed protein. Flow cytometry of spleen cells did not detect protein from mutant cells. (J:109720)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ly86 Mutation: 17 strains or lines available

References

• Original: J:109720 Nagai Y, et al., Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide. Blood. 2002 Mar 1;99(5):1699-705
• All: 11 reference(s)