Runx3^tm2Yg
Targeted Allele Detail

Summary

• Symbol: Runx3^tm2Yg
• Name: runt related transcription factor 3; targeted mutation 2, Yoram Groner
• MGI ID: MGI:3641439
• Synonyms: Runx3^deltaV, Runx3^VWRPY-
• Gene: Runx3 Location: Chr4:134847963-134905301 bp, + strand Genetic Position: Chr4, 67.19 cM
• Alliance: Runx3^tm2Yg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:109584
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting vector was designed such that the codons of the five C-terminal amino acids, VWRPY, were changed to the stop codon UAG and to codons encoding substituted amino acids designed to create a new NotI site. A loxP-flanked neo was included in intron 5 and was subsequently removed via cre-mediated excision. RT-PCR and Western blot analyses demonstrated expression of the mutant transcript and protein, respectively. (J:109584)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx3 Mutation: 27 strains or lines available

References

• Original: J:109584 Yarmus M, et al., Groucho/transducin-like Enhancer-of-split (TLE)-dependent and -independent transcriptional regulation by Runx3. Proc Natl Acad Sci U S A. 2006 May 9;103(19):7384-9
• All: 5 reference(s)