Tg(Pgk2-cre)24Shb
Transgene Detail

Summary

• Symbol: Tg(Pgk2-cre)24Shb
• Name: transgene insertion 24, Seiji Shioda
• MGI ID: MGI:3641577
• Synonyms: Tg(Pgk2-Cre)24Shb
• Transgene: Tg(Pgk2-cre)24Shb Location: unknown
• Alliance: Tg(Pgk2-cre)24Shb page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Pgk2-cre)24Shb expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Pgk2

• Mutation details: Nucleotides -1404 through +61 of the spermatocyte-specific Pgk2 promoter region were joined to the cDNA encoding Cre recombinase followed by a rabbit globin polyadenylation signal. Expression of cre mRNA in testes was demonstrated by RT-PCR analysis. Double transgenic progeny of a cross of mice with this transgene to animals carrying a loxP-flanked EGFP reporter gene exhibit EGFP fluorescence in spermatocytes and spermatids, but not in spermatogonia or somatic cells of the testis. (J:110868)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pgk2 (species not specified) Summary of all recombinase alleles driven by Pgk2.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Two transgenic mice derived from injected oocytes transmitted the transgene to their progeny. The resultant transgenic mouse lines were designated 3 and 24. Because Cre-mediated recombination sometimes failed to occur in double transgenic progeny of line 3, only line 24 was used.

References

• Original: J:110868 Kido T, et al., The testicular fatty acid binding protein PERF15 regulates the fate of germ cells in PERF15 transgenic mice. Dev Growth Differ. 2005 Jan;47(1):15-24
• All: 9 reference(s)