Tg(CAG-cat,-EGFP)39Miya
Transgene Detail

Summary

• Symbol: Tg(CAG-cat,-EGFP)39Miya
• Name: transgene insertion 39, Jun-ichi Miyazaki
• MGI ID: MGI:3652575
• Synonyms: CAG-CAT-EGFP, CAG-CAT-EGFP reporter transgene, CAG-CAT-EGFP transgene, eGFP^LSL, Flox-CAT-EGFP, line 39, LSL-GFP, Tg(ACTB-cat,-EGFP)39Miya, Tg(CAG-CAT-EGFP)39Miya
• Transgene: Tg(CAG-cat,-EGFP)39Miya Location: unknown
• Alliance: Tg(CAG-cat,-EGFP)39Miya page

Transgene origin

• Strain of Origin: (C57BL/6NJcl x DBA/2NJcl)F2

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: This Cre recombinase reporter transgene employs the "CAG promoter," which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene. The promoter is separated from the EGFP coding sequence, with a rabbit globin polyadenylation signal, by a loxP-flanked chloramphenicol acetyltransferase gene (cat) with the SV40 small t intron and polyadenylation signal. The cat gene is efficiently excised by Cre recombinase, permitting EGFP expression under control of the beta-actin promoter. (J:83047)

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Of nine transgenic lines whose offspring from crosses with cre transgenic mice fluoresced, four - numbered 20, 39 and 42, with estimated transgene copy numbers of 22, 21 and 13, respectively - were propagated. Line 39 was used in later experiments because of the strong fluorescence of the peripheral lymphocytes of its cre-transgenic progeny.

References

• Original: J:83047 Kawamoto S, et al., A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre-mediated recombination. FEBS Lett. 2000 Mar 31;470(3):263-8
• All: 149 reference(s)