Dapp1^{Gt(OST111213)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Dapp1^{Gt(OST111213)Lex}
• Name: dual adaptor for phosphotyrosine and 3-phosphoinositides 1; gene trap OST111213, Lexicon Genetics
• MGI ID: MGI:3652582
• Synonyms: Bam32^-, Dapp1^Gt708Lex
• Gene: Dapp1 Location: Chr3:137636768-137687306 bp, - strand Genetic Position: Chr3, 63.98 cM, cytoband H2
• Alliance: Dapp1^{Gt(OST111213)Lex} page

Mutation origin

• Mutant Cell Line: OST111213
• Germline Transmission: Earliest citation of germline transmission: J:110009
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The gene trap vector integrated between the two exons that contribute equally to the complete SH2 domain. A truncation was predicted in the middle of the SH2 domain at amino acid 74, which would result in a nonfunctional SH2 domain. Northern blot and RT-PCR confirmed loss of wild-type transcript in mutants. (J:110009)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST111213	CG530057 ; (MGI Seq Detail)	3' RACE	Chr7:130479461-130479624 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  25 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dapp1 Mutation: 28 strains or lines available

References

• Original: J:110009 Fournier E, et al., The B cell SH2/PH domain-containing adaptor Bam32/DAPP1 is required for T cell-independent II antigen responses. Curr Biol. 2003 Oct 28;13(21):1858-66
• All: 3 reference(s)