Tg(CLCNKB-WNK4)1Suc
Transgene Detail

Summary

• Symbol: Tg(CLCNKB-WNK4)1Suc
• Name: transgene insertion 1, Shinichi Uchida
• MGI ID: MGI:3652731
• Synonyms: Tg(CLCNKB-WNK4^D564A)
• Transgene: Tg(CLCNKB-WNK4)1Suc Location: unknown
• Alliance: Tg(CLCNKB-WNK4)1Suc page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CLCNKB-WNK4)1Suc expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  WNK4 (65266)

  Wnk4	(MGI:1917097)

• Mutation details: An 11-kb human genomic DNA fragment that includes the promoter and first intron of the chloride channel Kb (CLCNKB) gene, extending from the 3' end of the CLCNKA gene to immediately upstream of the translation start site of CLCNKB, is joined to the 5' end of a cDNA encoding a C-terminal hemagglutinin (HA) tagged, mutant human WNK lysine deficient protein kinase 4 with alanine replacing aspartic acid at amino acid position 564 (D564A), which resides in a highly conserved segment distal to the first coil domain of the protein, and followed by an SV40 polyadenylation signal. Immunoblot analysis of kidney homogenate using anti-HA antibody demonstrated expression in the kidney. Immunohistochemical analysis localized kidney expression of the mutant protein to the distal nephrons of the cortex and outer medulla, the thick ascending limb of Henley's loop (TAL) and, variably, cells of the distal tubules. In the last, signal is localized to the cytoplasm, while in TAL it is concentrated at the apical membrane. (J:110994)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Two transgenic founder mice were identified and "bred to establish the transgenic line."

Although the D564A mutation of WNK4 is associated with pseudohypoaldosteronism type II (PHA2) [OMIM 145260], mice with this transgene were not developed as a model for this disease. The kidney expression patterns of CLCNKB and wild-type WNK4 are not identical, and transgenic mice are neither hypertensive nor hyperkalemic.

References

• Original: J:110994 Yamauchi K, et al., Apical localization of renal K channel was not altered in mutant WNK4 transgenic mice. Biochem Biophys Res Commun. 2005 Jul 8;332(3):750-5
• All: 1 reference(s)