Foxn1^tm2Tbo
Targeted Allele Detail

Summary

• Symbol: Foxn1^tm2Tbo
• Name: forkhead box N1; targeted mutation 2, Thomas Boehm
• MGI ID: MGI:3653425
• Synonyms: Foxn1^SA2
• Gene: Foxn1 Location: Chr11:78248403-78277384 bp, - strand Genetic Position: Chr11, 46.74 cM
• Alliance: Foxn1^tm2Tbo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:110046
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele was created to be a conditional mutant whereas insertion of the targeting vector interrupts expression of the locus and expression of cre recombinase restores the function of the endogenous gene. The vector contains a cassette containing 2 copies of a 605 bp sequence, each containing a splice acceptor and polyA signal, and a hygromycin resistance gene. The cassette was flanked by loxP sites and inserted into intron 6 along with a 555 bp deletion of intronic sequence to facilitate the detection of cre-mediated remoal of the SA2-hyg cassette. (J:110046)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxn1 Mutation: 106 strains or lines available

References

• Original: J:110046 Bleul CC, et al., Formation of a functional thymus initiated by a postnatal epithelial progenitor cell. Nature. 2006 Jun 22;441(7096):992-6
• All: 2 reference(s)