Rr163^tm1Lfdn
Targeted Allele Detail

Summary

• Symbol: Rr163^tm1Lfdn
• Name: regulatory region 163; targeted mutation 1, Laurent Ferradini
• MGI ID: MGI:3653474
• Synonyms: Igk^tm1Lfdn
• Gene: Rr163 Location: Chr6:70699437-70699506 bp Genetic Position: Chr6, Syntenic
• Alliance: Rr163^tm1Lfdn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:31727
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: A vector was designed to replace 8 and 9 bp of the KI and KII sites (palindromic sequences, located in the intron between the Igkv and Igkj gene segments, involved in Igk cluster recombination), respectively. The sequence of KI was mutated from TCCACGCATGCTTGGA to TGTACGCTGCTGATGA and KII was mutated from TCCTCTTCAGTGAGGAG to AGCTCTTCTAGACTGA. A neomycin resistance gene cassette was deleted via cre-mediated recombination. (J:31727)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr163 Mutation: 0 strains or lines available

References

• Original: J:31727 Ferradini L, et al., Rearrangement-enhancing element upstream of the mouse immunoglobulin kappa chain J cluster. Science. 1996 Mar 8;271(5254):1416-20
• All: 1 reference(s)