Atp2a2^tm1Fwuy
Targeted Allele Detail

Summary

• Symbol: Atp2a2^tm1Fwuy
• Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; targeted mutation 1, Frank Wuytack
• MGI ID: MGI:3653502
• Synonyms: Serca2a^-, SERCA2^b
• Gene: Atp2a2 Location: Chr5:122591576-122640288 bp, - strand Genetic Position: Chr5, 62.38 cM, cytoband F
• Alliance: Atp2a2^tm1Fwuy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:111247
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was used to insert an inactivated muscle-specific 5' donor splice site and a strong polyA site. A neomycin resistance cassette replaced untranslated exons 23 and 24, and the muscle-specific exon 25. The resulting allele lacked expression of the protein in the heart and prevented its replacement by the b isoform. RNase protection assay and Western blot failed to detect transcript and protein, respectively, in mutants. (J:111247)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atp2a2 Mutation: 76 strains or lines available

References

• Original: J:111247 Ver Heyen M, et al., Replacement of the muscle-specific sarcoplasmic reticulum Ca(2+)-ATPase isoform SERCA2a by the nonmuscle SERCA2b homologue causes mild concentric hypertrophy and impairs contraction-relaxation of the heart. Circ Res. 2001 Oct 26;89(9):838-46
• All: 6 reference(s)