Zfp423^Gt(XH542)Byg
Gene trapped Allele Detail

Summary

• Symbol: Zfp423^Gt(XH542)Byg
• Name: zinc finger protein 423; gene trap XH542, BayGenomics
• MGI ID: MGI:3653507
• Synonyms: Zfp423^{Gt(pGT1Lxf)5Byg}
• Gene: Zfp423 Location: Chr8:88388438-88686223 bp, - strand Genetic Position: Chr8, 42.29 cM, cytoband C4
• Alliance: Zfp423^Gt(XH542)Byg page

Mutation origin

• Mutant Cell Line: XH542
• Germline Transmission: Earliest citation of germline transmission: J:111316
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: Bay Genomics gene trap vector pGt1Lxf was randomly inserted 11kb downstream of exon 3 in intron 3 which is 75 kb in size. RNA for the wild-type allele was detected in the brain by RT-PCR along with a larger band in mice carrying the gene trap allele and lacking the wild-type allele (J:111316). An independent report indicated that this allele is hypomorphic and that residual low-level expression of a properly spliced transcript is found in homozygous mice (J:114758). (J:111316)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XH542	CC199695 ; (MGI Seq Detail)	5' RACE	Chr8:88586003-88685868 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  439 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp423 Mutation: 274 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:111316 Warming S, et al., Zfp423 is required for normal cerebellar development. Mol Cell Biol. 2006 Sep;26(18):6913-22
• All: 4 reference(s)