Kl^tm2Yin
Targeted Allele Detail

Summary

• Symbol: Kl^tm2Yin
• Name: klotho; targeted mutation 2, Yo-ichi Nabeshima
• MGI ID: MGI:3653671
• Synonyms: kl^-geo
• Gene: Kl Location: Chr5:150876072-150917282 bp, + strand Genetic Position: Chr5, 89.77 cM, cytoband G3
• Alliance: Kl^tm2Yin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:102218
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 681 bp fragment of exon 1 was replaced with a lacZ-neomycin fusion gene (betageo) and a floxed PGK-neo cassette. The floxed neo was removed by cre mediated recombination. LacZ expression is seen in a pattern similar to expression of the endogenous gene. Western blot analysis confirmed the absence of protein in homozygotes (J:102218)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kl Mutation: 53 strains or lines available

References

• Original: J:102218 Takeshita K, et al., Sinoatrial node dysfunction and early unexpected death of mice with a defect of klotho gene expression. Circulation. 2004 Apr 13;109(14):1776-82
• All: 1 reference(s)