Gabra1^tm3Geh
Targeted Allele Detail

Summary

• Symbol: Gabra1^tm3Geh
• Name: gamma-aminobutyric acid type A receptor subunit alpha 1; targeted mutation 3, Gregg E Homanics
• MGI ID: MGI:3654125
• Synonyms: HA-neo, SL
• Gene: Gabra1 Location: Chr11:42021766-42073757 bp, - strand Genetic Position: Chr11, 24.97 cM
• Alliance: Gabra1^tm3Geh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:110982
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The 3' end was modified to flank exons 9 and 10 wit loxP sites, insert a mutated duplication of exons 9 and 10 downstream of all alpha1 coding and regulatory sequences and insert a neo cassette flanked by frt sites. Exons 9 and 10 were fused together and intron 9 deleted in the vector. The knockin mutations for exon 9 were to change serine 270 to histidine and leucine 277 to alanine. The neo was subsequently removed via crossing with FLPe general deleter mice. (J:110982)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gabra1 Mutation: 48 strains or lines available

References

• Original: J:110982 Skvorak K, et al., Production of conditional point mutant knockin mice. Genesis. 2006 Jul;44(7):345-53
• All: 1 reference(s)