Tg(YACW408A5)1952Ricc
Transgene Detail

Summary

• Symbol: Tg(YACW408A5)1952Ricc
• Name: transgene insertion 1952, Andrea Riccio
• MGI ID: MGI:3655816
• Synonyms: YAC W408A5
• Transgene: Tg(YACW408A5)1952Ricc Location: unknown
• Alliance: Tg(YACW408A5)1952Ricc page

Transgene origin

• Strain of Origin: 129/Sv

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(YACW408A5)1952Ricc expresses 4 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Cdkn1c (MGI:104564)
  mouse	Kcnq1 (MGI:108083)
  mouse	Phlda2 (MGI:1202307)
  mouse	Slc22a18 (MGI:1336884)

• Mutation details: An 800kb yeast artificial chromosome (YAC) designated W408A5, which contained C57BL/6 distal chromosome 7 sequence from the Cars to Th gene, and including imprinting center(IC2 which contains Cdkn1c (P57) and Kcnq1) and is syntenic to human chromosome 11p15.5, was modified by addition of 2 copies of the Neo-selection cassette. The construct was introduced into 129/Sv ES cells by yeast spheroplast fusion. After maternal transmission of the transgene, most maternally expressed genes of the IC2 region (Phdla2, Slc22a18, Cdkn1c and Kcnq1) show 30-60% increased expression in the placenta relative to wild-type and equivalent or slightly reduced expression after paternal transmission. With paternal transmission, paternally expressed gene Kcnq1ot1 shows 2-fold increased expression over wild-type or after maternal transmission. Ascl2 expression (maternally expressed) is increased 2-fold after maternal or paternal transmission, while Tssc4 expression is increased 2-fold after maternal and by ~33% after paternal transmission. Imprinting of these 2 genes is not reproduced by the transgene. Expression of Cdkn1c and Kcnq1ot1 in the embryo is comparable to the placental expression (J:66461, J:96366)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:96366 Cerrato F, et al., The two-domain hypothesis in Beckwith-Wiedemann syndrome: autonomous imprinting of the telomeric domain of the distal chromosome 7 cluster. Hum Mol Genet. 2005 Feb 15;14(4):503-11
• All: 2 reference(s)