Gars1^{Gt(XM256)6Byg}
Gene trapped Allele Detail

Summary

• Symbol: Gars1^{Gt(XM256)6Byg}
• Name: glycyl-tRNA synthetase 1; gene trap 6, BayGenomics
• MGI ID: MGI:3655818
• Synonyms: Gars^XM256
• Gene: Gars1 Location: Chr6:55014992-55056485 bp, + strand Genetic Position: Chr6, 27.29 cM
• Alliance: Gars1^{Gt(XM256)6Byg} page

Mutation origin

• Mutant Cell Line: XM256
• Germline Transmission: Earliest citation of germline transmission: J:112221
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: A gene trap vector inserted into the second intron. The insertion site is 519bp downstream of exon 2. mRNA analysis showed that heterozygous mice express approximately half the amount of transcript compared to controls. (J:112221)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XM256	CC325212 ; (MGI Seq Detail)	5' RACE	Chr6:55023403-55023497 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  10 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gars1 Mutation: 42 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:112221 Seburn KL, et al., An Active Dominant Mutation of Glycyl-tRNA Synthetase Causes Neuropathy in a Charcot-Marie-Tooth 2D Mouse Model. Neuron. 2006 Sep 21;51(6):715-26
• All: 3 reference(s)