Tg(tetO-Aurkb,lacZ)41Kra
Transgene Detail

Summary

• Symbol: Tg(tetO-Aurkb,lacZ)41Kra
• Name: transgene insertion 41, Katya Ravid
• MGI ID: MGI:3656025
• Synonyms: CMV-Aurora-B/beta-gal, teto-AIM-1
• Transgene: Tg(tetO-Aurkb,lacZ)41Kra Location: unknown
• Alliance: Tg(tetO-Aurkb,lacZ)41Kra page

Transgene origin

• Strain of Origin: FVB/NJ

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: A vector pBI-G (Clontech #6150-1) was used to create this transgene. The vector contains a prokaryotic beta-galactosidase (lacZ) reporter gene under the control of the tetracycline-response element (TRE; containing seven direct repeats of the tetracycline operator (tetO) sequence fused to bidirectional cytomegalovirus (CMV) minimal promoter). The rat Aurora-kinase B cDNA (with N-terminal FLAG-tag sequence and 3' linked beta-globin gene) was placed downstream of the promoter and the purified construct was injected into fertilized FVB/NJ eggs to create the transgenic mice. (J:104239)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:104239 Nguyen HG, et al., Conditional overexpression of transgenes in megakaryocytes and platelets in vivo. Blood. 2005 Sep 1;106(5):1559-64
• All: 1 reference(s)