Mrtfa^tm1Eno
Targeted Allele Detail

Summary

• Symbol: Mrtfa^tm1Eno
• Name: myocardin related transcription factor A; targeted mutation 1, Eric N Olson
• MGI ID: MGI:3662669
• Synonyms: MRTFA^-
• Gene: Mrtfa Location: Chr15:80896482-81074937 bp, - strand Genetic Position: Chr15, 37.98 cM
• Alliance: Mrtfa^tm1Eno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:111412
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A vector was designed to delete a part of exon 9 and all of exon 10, which encode the basic, glutamine-rich and SAP domains. A lacZ was fused in frame with exon 9 and a neo cassette. RT-PCR demonstrated that the correct exons were deleted and that exon 8 was spliced to exon 12, thereby deleting the lacZ-neo cassette. The resulting truncated protein would be nonfunctional. (J:111412)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Mrtfa^tm1Eno/Mrtfa^tm1Eno	involves: 129S/SvEv * C57BL/6
cn2	Mrtfa^tm1Eno/Mrtfa^tm1Eno Mrtfb^tm2.1Eno/Mrtfb^tm2.1Eno Tg(GFAP-cre)25Mes/0	involves: 129 * 129S/SvEv * C57BL/6 * FVB/N
cx3	Mamstr^tm1.2Eno/Mamstr^tm1.2Eno Mrtfa^tm1Eno/Mrtfa^tm1Eno	involves: 129 * C57BL/6

Phenotypes:

Affected Systems	hm1	cn2	cx3
show or hide all annotated terms Sex:
behavior/neurological	√
galactostasis	√
cellular		√	√
increased neuron apoptosis		√
abnormal neuron differentiation		√
impaired fibroblast cell migration		√
abnormal neuronal migration		√
abnormal skeletal muscle satellite cell proliferation			√
endocrine/exocrine glands	√
abnormal involution of the mammary gland	√
abnormal mammary gland growth during lactation	√
abnormal mammary gland myoepithelium morphology	√
pale mammary gland	√
abnormal lactation	√
galactostasis	√
abnormal mammary gland myoepithelium physiology	√
increased mammary gland apoptosis	√
growth/size/body		√	√
decreased body weight		√	√
cachexia			√
integument	√
abnormal involution of the mammary gland	√
abnormal mammary gland growth during lactation	√
abnormal mammary gland myoepithelium morphology	√
pale mammary gland	√
abnormal lactation	√
galactostasis	√
abnormal mammary gland myoepithelium physiology	√
increased mammary gland apoptosis	√
mortality/aging		√	√
postnatal lethality, complete penetrance		√
perinatal lethality, incomplete penetrance			√
muscle			√
decreased skeletal muscle weight			√
muscular atrophy			√
abnormal muscle physiology			√
abnormal skeletal muscle satellite cell proliferation			√
impaired skeletal muscle regeneration			√
nervous system		√
increased neuron apoptosis		√
abnormal neuron differentiation		√
abnormal neuronal migration		√
absent corpus callosum		√
absent anterior commissure		√
decreased striatum size		√
abnormal hippocampus morphology		√
abnormal dentate gyrus morphology		√
abnormal hippocampus pyramidal cell layer		√
abnormal hippocampus neuron morphology		√
abnormal olfactory bulb morphology		√
abnormal rostral migratory stream morphology		√
abnormal postnatal subventricular zone morphology		√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mrtfa Mutation: 32 strains or lines available

References

• Original: J:111412 Li S, et al., Requirement of a myocardin-related transcription factor for development of mammary myoepithelial cells. Mol Cell Biol. 2006 Aug;26(15):5797-808
• All: 24 reference(s)