Glra2^tm1Clc
Targeted Allele Detail

Summary

• Symbol: Glra2^tm1Clc
• Name: glycine receptor, alpha 2 subunit; targeted mutation 1, Constance L Cepko
• MGI ID: MGI:3662671
• Gene: Glra2 Location: ChrX:163912013-164110389 bp, - strand Genetic Position: ChrX, 76.75 cM
• Alliance: Glra2^tm1Clc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:111414
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A vector was designed to replace exons 6 and 7 with an FRT-flanked PGK-neomycin cassette. These exons encode two transmembrane domains and a large portion of its ligand binding domain. Alternative splicing of exon 5 with exon 8 would result in an immediate premature stop codon and should lead to a nonsense-mediated degradation of the mRNA. RT-PCR, Northern blot and in situ hybridization assays were performed to confirm absence of transcript in mutants. (J:111414)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Glra2 Mutation: 9 strains or lines available

References

• Original: J:111414 Young-Pearse TL, et al., Characterization of mice with targeted deletion of glycine receptor alpha 2. Mol Cell Biol. 2006 Aug;26(15):5728-34
• All: 4 reference(s)