Ssbp3^Gt(XB276)Byg
Gene trapped Allele Detail

Summary

• Symbol: Ssbp3^Gt(XB276)Byg
• Name: single-stranded DNA binding protein 3; gene trap XB276, BayGenomics
• MGI ID: MGI:3663393
• Synonyms: Ssbp3^{Gt(pGT0pfs)7Byg}, Ssdp1¹²²
• Gene: Ssbp3 Location: Chr4:106768667-106906891 bp, + strand Genetic Position: Chr4, 49.9 cM, cytoband C7
• Alliance: Ssbp3^Gt(XB276)Byg page

Abnormal head development in Ssbp3^Gt(XB276)Byg/Ssbp3^Gt(XB276)Byg mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: XB276
• Germline Transmission: Earliest citation of germline transmission: J:111801
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: pGT0pfs Vector Type: gene trap
• Mutation details: A Beta-geo gene trap vector, pGTOpfs, was inserted into intron 5 of the locus. The first 122 amino acids were fused to the Beta-galactosidase-neomycin protein, thereby eliminating the proline rich stretch. Western blots demonstrate expression of chimeric protein composed of the endogenous N-terminal polypeptide fused to Beta-geo. (J:111801)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XB276	CC144416 ; (MGI Seq Detail)	5' RACE	Chr4:106769047-106866961 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  46 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ssbp3 Mutation: 41 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:111801 Enkhmandakh B, et al., The role of the proline-rich domain of Ssdp1 in the modular architecture of the vertebrate head organizer. Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11631-6
• All: 2 reference(s)