Pde10a^tm1Pfi
Targeted Allele Detail

Summary

• Symbol: Pde10a^tm1Pfi
• Name: phosphodiesterase 10A; targeted mutation 1, Pfizer, Ltd
• MGI ID: MGI:3663426
• Synonyms: Pde10^-, Pde10a^tm1Jasi
• Gene: Pde10a Location: Chr17:8744204-9205480 bp, + strand Genetic Position: Chr17, 4.96 cM
• Alliance: Pde10a^tm1Pfi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:111662
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: DBA/1LacJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo vector was designed to replace exons 14-16, corresponding to bases 1499-1861 of the coding region. Sequence analysis revealed that the neo cassette was spliced out. The resulting transcript encoded an in-frame deletion of 126 amino acids at the N-terminus of the catalytic domain. This terminus contained one of the two divalent zinc binding sites. Since the deletion was in-frame, a mutant protein of approximately 75 kDa could be produced. (J:111662)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pde10a Mutation: 61 strains or lines available

References

• Original: J:111662 Siuciak JA, et al., Genetic deletion of the striatum-enriched phosphodiesterase PDE10A: evidence for altered striatal function. Neuropharmacology. 2006 Aug;51(2):374-85
• All: 4 reference(s)