Tg(Cd8b1)46Erob
Transgene Detail

Summary

• Symbol: Tg(Cd8b1)46Erob
• Name: transgene insertion 46, Ellen Robey
• MGI ID: MGI:3663518
• Synonyms: dnlyt3
• Transgene: Tg(Cd8b1)46Erob Location: unknown
• Alliance: Tg(Cd8b1)46Erob page

Transgene origin

• Strain of Origin: C57BL/6 x CBA/J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Cd8b1)46Erob expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Cd8b1 (MGI:88347)

• Mutation details: Site directed mutagenesis was used to change the Arg-181 of a Cd8b1 gene fragment to a stop codon. This fragment replaced the corresponding sequence in a genomic fragment of the Cd8b1 gene. A 5 kb fragment with the human CD2 locus-controlling region downstream of the mutated gene was ligated to the genomic clone and the entire construct was injected into embryos. A founder with ~20 copies/diploid genome, designated dnlyt3, was used for analysis. "CD8b1" differs from the "CD8b2" allele normally expressed in the C57BL/6 mouse strain. Monoclonal antibodies can distinguish the endogenous protein from the transgene product in hemizygous transgenic mice. (J:110916)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

"CD8b1" differs from the "CD8b2" allele normally expressed in the C57BL/6 mouse strain. Monoclonal antibodies can distinguish the endogenous protein from the transgene product in hemizygous transgenic mice.

References

• Original: J:112749 Itano A, et al., A role for the cytoplasmic tail of the beta chain of CD8 in thymic selection. Immunity. 1994 Jul;1(4):287-90
• All: 2 reference(s)