Hck^tm1Ern
Targeted Allele Detail

Summary

• Symbol: Hck^tm1Ern
• Name: hemopoietic cell kinase; targeted mutation 1, Matthias Ernst
• MGI ID: MGI:3663799
• Synonyms: HckCA, Hck^F, Hck^Y499F
• Gene: Hck Location: Chr2:152950388-152993361 bp, + strand Genetic Position: Chr2, 75.41 cM
• Alliance: Hck^tm1Ern page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:111432
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Constitutively active)
• Mutation: Single point mutation
• Mutation details: A targeting vector was designed to introduce the tyrosine 499 to phenylalanine substitution (Y499F) and a stop cassette into exon 13. An IRES-neomycin was included in the vector. RT-PCR confirmed the mutated sequence. Western blot detected reduced levels of both isoforms created by the locus. (J:111432)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Hck Mutation: 31 strains or lines available

References

• Original: J:111432 Ernst M, et al., Constitutive activation of the SRC family kinase Hck results in spontaneous pulmonary inflammation and an enhanced innate immune response. J Exp Med. 2002 Sep 2;196(5):589-604
• All: 2 reference(s)