Tg(Myh6-Ngfb)4Fed
Transgene Detail

Summary

• Symbol: Tg(Myh6-Ngfb)4Fed
• Name: transgene insertion 4, Howard J Federoff
• MGI ID: MGI:3664113
• Synonyms: MHC-NGF, Tg(Ngfb)1Fed
• Transgene: Tg(Myh6-Ngfb)4Fed Location: unknown
• Alliance: Tg(Myh6-Ngfb)4Fed page

Transgene origin

• Strain of Origin: C3HeB/FeJ

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-Ngfb)4Fed expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  mouse

  Ngf (MGI:97321)

  Transgene codes for a hybrid or fusion protein of mouse and rat Ngf.

  rat

  Ngf (310738)

  Ngf	(MGI:97321)

• Mutation details: This transgene consists of a 654-bp fragment of rat alpha-myosin heavy chain promoter inserted upstream of a chimeric Ngfb mini-gene containing noncoding exon 1 and a portion of intron 1 of the mouse Ngfb gene fused to sequences from intron 3 through exon 4 of the rat Ngfb gene. Northern blots analysis shows cardiac specific expression of Ngfb. Three lines were established (2-4), with line 4 containing 8-10 copies of the transgene and lines 2 and 3 containing between 15 and 20 copies. Line 4 is chosen as a representative line as it continues to exhibit the cardiac pathology due to hypomethylation while lines 2 and 3 are hypermethylated and show loss of the phenotype after propagation for about 10 generations. (J:25270)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:25270 Hassankhani A, et al., Overexpression of NGF within the heart of transgenic mice causes hyperinnervation, cardiac enlargement, and hyperplasia of ectopic cells. Dev Biol. 1995 May;169(1):309-21
• All: 4 reference(s)