Ncr1^tm1Oman
Targeted Allele Detail

Summary

• Symbol: Ncr1^tm1Oman
• Name: natural cytotoxicity triggering receptor 1; targeted mutation 1, Ofer Mandelboim
• MGI ID: MGI:3664443
• Synonyms: Ncr1^gfp
• Gene: Ncr1 Location: Chr7:4340723-4348163 bp, + strand Genetic Position: Chr7, 2.5 cM
• Alliance: Ncr1^tm1Oman page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:112394
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exons 5-7 with an IRES-GFP-PGK-neo. The neo was removed via cre-mediated recombination. Absence of endogenous transcript was confirmed by RT-PCR. Fluorescence is more intense after excision by Tg(Prm-cre)58Og (129/Sv background) or by Tg(Sp3-cre)93Knw (C57BL/6 background). When allele is made congenic on 129 or C57BL/6 background, immune system phenotype is not altered by removal of PGK-neo or by background. (J:112394)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ncr1 Mutation: 40 strains or lines available

References

• Original: J:112394 Gazit R, et al., Lethal influenza infection in the absence of the natural killer cell receptor gene Ncr1. Nat Immunol. 2006 May;7(5):517-23
• All: 55 reference(s)