Igkc^tm2(IGKC)Cgn
Targeted Allele Detail

Summary

• Symbol: Igkc^tm2(IGKC)Cgn
• Name: immunoglobulin kappa constant; targeted mutation 2, University of Cologne
• MGI ID: MGI:3664449
• Synonyms: CkappaR
• Gene: Igkc Location: Chr6:70703419-70703738 bp, + strand Genetic Position: Chr6, 32.04 cM, cytoband C
• Alliance: Igkc^tm2(IGKC)Cgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:113008
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert the human IGKC and a neo cassette such that the sequence containing a splice signal and 12 base pairs of endogenous sequence were replaced. The remainder of the locus was rendered nonfunctional. Flow cytometry demonstrated the expression of humanized proteins on B cell surfaces. (J:113008)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igkc Mutation: 9 strains or lines available

References

• Original: J:113008 Zou YR, et al., Generation of a mouse strain that produces immunoglobulin kappa chains with human constant regions. Science. 1993 Nov 19;262(5137):1271-4
• All: 3 reference(s)