Braf^tm1(ARAF)Urr
Targeted Allele Detail

Summary

• Symbol: Braf^tm1(ARAF)Urr
• Name: Braf transforming gene; targeted mutation 1, Ulf R Rapp
• MGI ID: MGI:3664469
• Synonyms: B-RAF^KIN
• Gene: Braf Location: Chr6:39580171-39702397 bp, - strand Genetic Position: Chr6, 18.43 cM
• Alliance: Braf^tm1(ARAF)Urr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:112971
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The human ARAF cDNA was introduced in frame with exon 3 of the locus. This exon is common to all three isoforms and harbors the beginning of the Ras binding domain. RT-PCR and Western blot confirmed the production of chimeric transcripts and protein from the locus. A floxed neo included in the vector did not interfere with expression of the protein. (J:112971)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Braf Mutation: 60 strains or lines available

References

• Original: J:112971 Camarero G, et al., Cortical migration defects in mice expressing A-RAF from the B-RAF locus. Mol Cell Biol. 2006 Oct;26(19):7103-15
• All: 1 reference(s)