BY367451^tm1Mdk
Targeted Allele Detail

Summary

• Symbol: BY367451^tm1Mdk
• Name: cDNA sequence, BY367451; targeted mutation 1, Michael D Koob
• MGI ID: MGI:3664539
• Synonyms: Atxn8os^tm1Mdk, Klhl1^flox
• Gene: BY367451 Location: Chr14:96794007-96794446 bp Genetic Position: Chr14, Syntenic
• Alliance: BY367451^tm1Mdk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:112937
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into the transcription start site of Klhl1, which is also the upstream region of the polyA signal of Klhl1as. A neo cassette flanked with 2 FRT sites and a 3' loxP site were inserted into the first intron of Klhl1, which is downstream of the transcription start site of Klhl1as. Crossing with mice that express FLP in the germline resulted in the removal of the neo, leaving exon 1 of Klhl1 flanked by loxP sites. (J:112937)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any BY367451 Mutation: 0 strains or lines available

References

• Original: J:112937 He Y, et al., Targeted deletion of a single Sca8 ataxia locus allele in mice causes abnormal gait, progressive loss of motor coordination, and Purkinje cell dendritic deficits. J Neurosci. 2006 Sep 27;26(39):9975-82
• All: 1 reference(s)